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技術文章
TISSUE CULTURE STOCK SOLUTIONS AND MEDIA
點擊次數：587 發布時間：2009-10-19
MS MEDIUM FOR ARABIDOPSIS
To 990 ml H2O add:Sucrose ........... 10.0 gMOPS .............. 0.5 gAgar .............. 8.0 gAdjust pH to 5.7 with 1 M KOH.Autoclave.When cooled add sterile stock solutions of:KNO3............... 20.0 ml 940 mM （95.00 g/L）NH4NO3............. 20.0 ml 1000 mM （80.04 g/L）KH2PO4............. 12.5 ml 100 mM （13.60 g/L）MgSO47H2O.......... 15.0 ml 100 mM （24.65 g/L）CaCl27H2O.......... 10.0 ml 300 mM （44.00 g/L）Glycine............ 2.67 ml 10 mMNicotinate......... 0.4 ml 10 mMThiamin............ 0.02 ml 10 mMPyridoxineHCl...... 0.24 ml 10 mMMyoinositol........ 5.5 ml 100 mMFeSO47H2O ......... 1.0 ml （2.780 g/100 ml）Na2EDTA ........... 1.0 ml 100 mM （3.720 g/100 ml）MnSO44H2O.......... 1.0 ml 100 mM （1.690 g/100 ml）ZnSO44H2O.......... 3.0 ml 10 mM （0.288 g/100 ml）H3BO3 ............. 1.0 ml 100 mM （0.618 g/100 ml）KI................. 0.5 ml 10 mM （0.166 g/100 ml）CuSO45H2O ......... 0.01 ml 10 mM （0.170 g/100 ml）Na2MoO42H2O ....... 0.1 ml 10 mM （0.242 g/100 ml）CoCl26H2O ......... 0.01 ml 10 mM （0.238 g/100 ml）B5 MEDIUM FOR ARABIDOPSIS
To 990 ml H2O add:Sucrose ........... 20.0 gMOPS .............. 0.5 gAgar .............. 8.0 gAdjust pH to 5.7 with 1 M KOH.Autoclave.When cooled add sterile stock solutions of:KNO3............... 26.3 ml 940 mM （95.00 g/L）NaH2PO4H2O......... 11.0 ml 100 mMMgSO47H2O.......... 10.0 ml 100 mM （24.65 g/L）（NH4）2SO3.......... 10.0 ml 100 mMCaCl27H2O.......... 3.3 ml 300 mM （44.00 g/L）Myoinositol........ 5.5 ml 100 mMThiamine........... 3.0 ml 10 mMNicotinate......... 0.8 ml 10 mMPyridoxineHCl...... 0.48 ml 10 mM10X Micronutrients. 10.0 ml （see below）Sequestrene 330 Fe. 10.0 ml （0.28 g/100 ml）For Callus Inducing Medium （CIM） add:2,4-D..................... 0.5 mlKinetin................... 0.05 mlFor Shoot Inducing Medium （SIM） add:IAA....................... 0.15 ml6-dimethylaminopurine..... 5.0 ml
10X Micronutrients （per 100 ml）MnSO44H2O.......... 0.6 ml 100 mM （1.690 g/100 ml）ZnSO44H2O.......... 10.07 ml 10 mM （0.288 g/100 ml）H3BO3 ............. 4.9 ml 100 mM （0.618 g/100 ml）KI................. 4.5 ml 10 mM （0.166 g/100 ml）CuSO45H2O ......... 2.3 ml 10 mM （0.170 g/100 ml）Na2MoO42H2O ....... 1.0 ml 10 mM （0.242 g/100 ml）CoCl26H2O ......... 1.0 ml 10 mM （0.238 g/100 ml）